Journal: Frontiers in Immunology

Article Title: Human Placental Trophoblasts Infected by Listeria monocytogenes Undergo a Pro-Inflammatory Switch Associated With Poor Pregnancy Outcomes

doi: 10.3389/fimmu.2021.709466

Figure Lengend Snippet: Top 50 Induced Genes.

Article Snippet: IFNL1, IFNL2, and IFNL3 ELISAs were performed using the human IL-29 (DY7246), IL-28A (DY1587), and IL-28B (DY5259) DuoSet ELISA kits (R&D Systems) per the manufacturer’s instructions.

Techniques:

Journal: JCI Insight

Article Title: A cytotoxic anti-IL-3Rα antibody targets key cells and cytokines implicated in systemic lupus erythematosus

doi: 10.1172/jci.insight.86131

Figure Lengend Snippet: (A) IFN-α production, as measured by ELISA, in response to TLR4, TLR7, and TLR9 stimulation following CSL362 or isotype control pretreatment of healthy and SLE donor (n = 3 each) peripheral blood mononuclear cells (PBMCs). (B) Expression of 11 IFN-inducible genes (IFI44L, IFIT1, IFIT3, IRF7, ISG15, MX1, MX2, OAS1, OAS2, SERPING1, and XAF1), as determined by qPCR, in response to TLR1–9 agonist stimulation following CSL362 or isotype control pretreatment in healthy donor PBMCs (n = 4). (C) IFN-α production, as determined by ELISA, from healthy (n = 2) and SLE (n = 2) donor PBMCs that were depleted either of plasmacytoid dendritic cells (pDCs) or basophils and stimulated with TLR7 (imiquimod) or TLR9 (CpG C) agonist. Data are expressed as mean ± SEM, *P < 0.05 (Mann Whitney test).

Article Snippet: Type III IFN levels in supernatant were assessed with the DIY Human IFN lambda 3/1/2 (IL-28B/29/28A) ELISA kit (PBL Assay Science, catalog 61840).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Expressing, MANN-WHITNEY

Journal: JCI Insight

Article Title: A cytotoxic anti-IL-3Rα antibody targets key cells and cytokines implicated in systemic lupus erythematosus

doi: 10.1172/jci.insight.86131

Figure Lengend Snippet: (A) IFN-inducible gene expression, expressed as a single gene score, in SLE (n = 31) and healthy (n = 35) donor whole blood. Gene score represents the average log2 fold change in expression of 11 IFN-inducible genes (IFI44L, IFIT1, IFIT3, IRF7, ISG15, MX1, MX2, OAS1, OAS2, SERPING1, and XAF1) compared with a universal healthy donor. (B) IFN-α production from SLE (n = 9), autoimmune (n = 12), and healthy (n = 11) donor peripheral blood mononuclear cells (PBMCs) and (C) IFN-inducible gene expression, expressed as a single gene score for SLE (n = 10) and healthy (n = 9) donor PBMCs stimulated with TLR9 agonist (CpG C), following CSL362, Fab′CSL362 (B, only) or isotype control pretreatment. Gene score represents the average log2 fold change in expression of 11 IFN-inducible genes (IFI44L, IFIT1, IFIT3, IRF7, ISG15, MX1, MX2, OAS1, OAS2, SERPING1, and XAF1) for each treatment compared with no treatment. (D) IFN production (n = 3) from healthy donor PBMCs pretreated with CSL362 or isotype control and then stimulated with 50% SLE serum containing high anti-dsDNA antibody levels. The level of IFN-α detectable in the serum alone (no PBMC control) is shown in the “serum alone” condition. (E) IFN-inducible gene expression in healthy donor PBMCs stimulated with 50% SLE serum containing low or medium anti-dsDNA antibody levels, following CSL362 or isotype control pretreatment. IFN-inducible gene expression determined by qPCR, IFN-α production determined by ELISA. Data are expressed as mean ± SEM, *P < 0.05 (Mann Whitney test).

Article Snippet: Type III IFN levels in supernatant were assessed with the DIY Human IFN lambda 3/1/2 (IL-28B/29/28A) ELISA kit (PBL Assay Science, catalog 61840).

Techniques: Gene Expression, Expressing, Control, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: JCI Insight

Article Title: A cytotoxic anti-IL-3Rα antibody targets key cells and cytokines implicated in systemic lupus erythematosus

doi: 10.1172/jci.insight.86131

Figure Lengend Snippet: (A) IFN-α production in response to TLR9 (CpG C) or TLR7 (imiquimod) stimulation of healthy donor peripheral blood mononuclear cells (PBMCs) (n = 2) and (B) expression of 11 IFN-inducible genes (IFI44L, IFIT1, IFIT3, IRF7, ISG15, MX1, MX2, OAS1, OAS2, SERPING1, and XAF1) in response to TLR1–9 stimulation in healthy donors (n = 4), following pretreatment with CSL362, isotype control, or 2 anti-IFN-α mAbs. IFN-inducible gene expression determined by qPCR, IFN-α production determined by ELISA. Data are expressed as mean ± SEM, P < 0.05 (Mann Whitney test). (C) Expression of type I (IFNA, IFNB1, IFNW, IFNE) and type III (IFNL1, IFNL2, IFNL3) IFN subtypes in response to TLR9 or TLR7 stimulation in healthy donors (n = 6), as assessed by RNA sequencing of isolated pDCs. Type II IFN (IFNG) was not differentially expressed. (D) TLR7- and TLR9-stimulated IFN-λ (TLR9 — n = 3 healthy, n = 2 SLE; TLR7 — n = 3 healthy and n = 3 SLE) and IFN-γ (n = 9 healthy, n = 6 SLE) production, following pretreatment with CSL362 or isotype control. IFN-λ and IFN-γ production determined by ELISA. Data are expressed as mean ± SEM, *P < 0.05 (Mann Whitney test).

Article Snippet: Type III IFN levels in supernatant were assessed with the DIY Human IFN lambda 3/1/2 (IL-28B/29/28A) ELISA kit (PBL Assay Science, catalog 61840).

Techniques: Expressing, Control, Gene Expression, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, RNA Sequencing, Isolation

Journal: JCI Insight

Article Title: A cytotoxic anti-IL-3Rα antibody targets key cells and cytokines implicated in systemic lupus erythematosus

doi: 10.1172/jci.insight.86131

Figure Lengend Snippet: (A) Serum concentration of CSL362, determined by ELISA at various time points following s.c. administration of CSL362 (at doses of 1, 10, and 30 mg/kg) to cynomolgus macaques. (B) Representative flow cytometric analysis showing plasmacytoid dendritic cells (pDCs) (Lin1–, HLA-DR+, BDCA2+, CD123+) and basophils (IgE+, CD123+) at baseline and at 6 hours and 36 days after single dose of CSL362. At various time points following s.c. administration of CSL362, (C) pDCs and (D) basophils, in peripheral blood, expressed as a percentage of baseline (pretreatment), as determined by flow cytometry. (E) Expression of 6 IFN-inducible genes (IFI35, IFIT1, IRF7, MX1, MX2, OAS1) as a single gene score in peripheral blood mononuclear cells cultured with TLR9 agonist (CpG), as determined by qPCR. The gene score represents the average log2 fold change in the 6 IFN-inducible genes over unstimulated control. Data are expressed as mean ± SEM for n = 3 animals per dosing group.

Article Snippet: Type III IFN levels in supernatant were assessed with the DIY Human IFN lambda 3/1/2 (IL-28B/29/28A) ELISA kit (PBL Assay Science, catalog 61840).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Expressing, Cell Culture, Control

Journal: Arthritis Research & Therapy

Article Title: Interferon-λ3/4 genetic variants and interferon-λ3 serum levels are biomarkers of lupus nephritis and disease activity in Taiwanese

doi: 10.1186/s13075-018-1683-z

Figure Lengend Snippet: Association of IFNL3/4 SNPs with SLE susceptibility

Article Snippet: An IFNL3 ELISA kit (catalog no. CSB-E13296h; CUSABIO, College Park, MD, USA) was used to measure serum IFNL levels of SLE patients according to the manufacturer’s instructions.

Techniques: Positive Control, Control

Journal: Arthritis Research & Therapy

Article Title: Interferon-λ3/4 genetic variants and interferon-λ3 serum levels are biomarkers of lupus nephritis and disease activity in Taiwanese

doi: 10.1186/s13075-018-1683-z

Figure Lengend Snippet: Association of IFNL3/4 SNPs with lupus nephritis among SLE patients

Article Snippet: An IFNL3 ELISA kit (catalog no. CSB-E13296h; CUSABIO, College Park, MD, USA) was used to measure serum IFNL levels of SLE patients according to the manufacturer’s instructions.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Interferon-λ3/4 genetic variants and interferon-λ3 serum levels are biomarkers of lupus nephritis and disease activity in Taiwanese

doi: 10.1186/s13075-018-1683-z

Figure Lengend Snippet: Detection of IL-28B (IFNL3) and IL-28 receptor alpha (IL-28RA) in kidney tissue of lupus patients with nephritis. IFNL3 detected on parietal cells (red arrow), podocytes (yellow arrows), and tubular cells (blue arrows) of kidney from lupus patient with minimal change disease ( a ) and lupus patient with class IV proliferative nephritis ( b ). IL-28RA expressed in parietal cells (red arrow), podocytes (yellow arrows), and tubular cells (blue arrows) of kidney from patient with minimal change disease ( c ) and lupus patient with class IV proliferative nephritis ( d )

Article Snippet: An IFNL3 ELISA kit (catalog no. CSB-E13296h; CUSABIO, College Park, MD, USA) was used to measure serum IFNL levels of SLE patients according to the manufacturer’s instructions.

Techniques: